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KMID : 1098420230310050304
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Korean Journal of Medicinal Crop Science
2023 Volume.31 No. 5 p.304 ~ p.315
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Development of Genomic Simple Sequence Repeat Markers andVariety Discrimination in Salvia miltiorrhiza Bunge
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Kim Ei-Hyun
Ma Kyung-Ho
Lee Jeong-Hoon
Jeong Jin-Tae
Han Jong-Won
Chung Jong-Wook
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Abstract
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Background: Salvia miltiorrhiza Bunge, of the Lamiaceae family, is a medicinal plant. This study aimed to develop polymorphic simple sequence repeat (SSR) markers for analyzing the genetic diversity and distinguishing between varieties of S. miltiorrhiza.
Methods and Results: Using whole genome resequencing data, 50 SSR markers were designed for S. miltiorrhiza. Of these, 41 polymorphic markers were selected and utilized to assess 44 S. miltiorrhiza accessions. A total of 346 alleles were detected (2 to 17 per locus, averaging 8.4). Major allele frequency ranged from 0.19 to 0.83 (average 0.47), observed heterozygosity ranged from 0 to 0.60 (average 0.22), and polymorphic information content ranged from 0.29 to 0.87 (average 0.64). Among the 41 SSR markers, 18 were effective for distinguishing varieties, particularly S. miltiorrhiza ‘Dasan’, ‘Gosan’, and 'Hongdan' varieties.
Conclusions: The SSR markers developed in this study could be effectively used for variety discrimination, genetic diversity analysis, and population genetics studies for breeding in S. miltiorrhiza.
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KEYWORD
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Salvia miltiorrhiza Bunge, Genetic Diversity, Simple Sequence Repeats Marker Development, Variety Discrimination
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